You can also advance through the “Stack” of images in the video, frame by frame, by clicking on the right and left arrows surrounding the scroll bar across the bottom of the image. By clicking play, you should be able to watch the video on a loop. In the new window, tick the ‘Convert to Grayscale’ and ‘Flip Vertical’ boxes, and then click ‘OK’. ‘5umBeads_ 40X_Tilted.AVI’), and click ‘Open.’ A new window will appear. In this new window, select the AVI file that you wish to open (e.g. Once ImageJ is open, go to the ‘File’ menu and select ‘Import’, ‘AVI…’. Restart ImageJ.)ĭouble click on the ImageJ icon or find ImageJ in your Program Files. Drag the file from the ‘Downloads’ folder into the ‘Stacks’ folder. Also open the ‘ImageJ’ folder (Computer/Program Files/ImageJ), the ‘plugins’ folder inside it, and the ‘Stacks’ folder inside that. Open the ‘Downloads’ folder and find the file. (On a Windows machine, the following steps should work: Click on the Manual_Tracking.class link. ) To install this on your computer, you will need to click on Manual_Tracking.class and save it to the ImageJ Plugins folder-specifically, the Stacks folder. You will need the Manual Tracking plugin (under “Stacks”). (You can probably find this information on a Windows machine by looking in the hard disk, under the ‘Windows’ file, for the System folder (either ‘System32’ or ‘System64’) that contains the Java code.) Follow the installation instructions generated by your computer or provided at the website (linked above) for your operating system. Regardless of your platform, you will need to know whether the Java you are using is 32-bit or 64-bit.
Go to: and download the program appropriate for the operating system that you are using.
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